| Place | Performance | Athlete | Team |
| 1 | 3:55.75 | Q: NTANGA - LOUISA | GAUTENG |
| 2 | 3:56.51 | Q: MLAMBO - ZUZOKUHLE | MPUMALANGA |
| 3 | 3:57.92 | Q: VAN DYK - CHELSEA | GAUTENG |
| 4 | 3:58.10 | Q: HARRIS - XANTE | GAUTENG |
| 5 | 4:01.24 | Q: MOKOENA - MASENTLE | FREESTATE |
| 6 | 4:03.54 | q: DE BEER - LUMARI | WESTERN CAPE |
| 7 | 4:10.20 | q: COETZEE - KARIEN | WESTERN CAPE |