| Place | Performance | Athlete | Team |
| 1 | 2:26.09 | Q: MAMETJA - KGOTLELELO | LIMPOPO |
| 2 | 2:26.36 | Q: KRUGER - LIESA | FREESTATE |
| 3 | 2:26.42 | Q: VAN DER MERWE - SABELLA | GAUTENG |
| 4 | 2:27.02 | q: HOLTSHAUZEN - DANICA | MPUMALANGA |
| 5 | 2:29.13 | q: NELSON - HEYLINN | GAUTENG |
| 6 | 2:29.60 | q: DE JONG - TAYLIN | GAUTENG |
| 7 | 2:29.75 | q: THOMATHOS - TATIANA | WESTERN CAPE |
| 8 | 2:39.95 | -: RADEBE - ELIHLE | KWAZULU-NATAL |
| 9 | 2:41.27 | -: KHUMALO - THEMBELA | KWAZULU-NATAL |