| Place | Performance | Athlete | Team |
| 1 | 2:24.08 | Q: HOMAN - CELIA | GAUTENG |
| 2 | 2:24.10 | Q: VAN DER MERWE - LIANE | MPUMALANGA |
| 3 | 2:25.79 | Q: DE BEER - CAPRIVI | FREESTATE |
| 4 | 2:27.27 | q: SWANEPOEL - LEE-ANN | NORTHWEST PROVINCE |
| 5 | 2:27.68 | q: KLEYNHANS - MEGAN | GAUTENG |
| 6 | 2:27.78 | q: MEYER - LEIGH-ANNE | FREESTATE |
| 7 | 2:30.10 | -: ENGELBRECHT - GEMMA | GAUTENG |
| 8 | 2:36.52 | -: GILIVITHI - ASANELE | EASTERN CAPE |