| Place | Performance | Athlete | Team |
| 1 | 23.27 | Q: MOJAPELO - KWENA | GAUTENG |
| 2 | 23.70 | Q: PHILI - MHOLI | KWAZULU-NATAL |
| 3 | 25.03 | -: MKHUPHENI - SIPHIWE | EASTERN CAPE |
| 4 | 25.04 | -: PHALANE - MOGUDI LASI | MPUMALANGA |
| 5 | 25.43 | -: TIETIES - Kenro | NORTHERN CAPE |
| 6 | 26.06 | -: NKUNA - JOY | MPUMALANGA |
| 7 | -: NDLOVU - VUYOLWETHU | GAUTENG |