| Place | Performance | Athlete | Team |
| 1 | 25.98 | Q: FOURIE - CHANE | GAUTENG |
| 2 | 26.61 | Q: BOTMA - CHENADE | MPUMALANGA |
| 3 | 27.05 | -: JORDAAN - DINIKE | NORTHWEST PROVINCE |
| 4 | 27.16 | -: VAN DER MERWE - LIZE | MPUMALANGA |
| 5 | 27.52 | -: JV RENSBURG - ELOUISE | GAUTENG |
| 6 | 28.03 | -: ELS - ANE | MPUMALANGA |
| 7 | 28.46 | -: SIMONSE - Taryn | NORTHERN CAPE |
| 8 | 28.69 | -: ERNS - PRECIOUS | EASTERN CAPE |