| Place | Performance | Athlete | Team |
| 1 | 25.84 | Q: MAAKE - MOKGADI KGAUGELO | LIMPOPO |
| 2 | 26.41 | Q: JANSE - NICOLENE | WESTERN CAPE |
| 3 | 26.77 | -: KLEINSMITH - AMERIZE | GAUTENG |
| 4 | 26.78 | -: VAN ROOYEN - AURILEA | WESTERN CAPE |
| 5 | 27.06 | -: MANDERS - JAYDEN | NORTHWEST PROVINCE |
| 6 | 27.97 | -: SAMPSON - Rukaiya | NORTHERN CAPE |
| 7 | 28.38 | -: LEROBANE - BUSISIWE | LIMPOPO |
| 8 | 28.64 | -: DEYI - HAVILLAH | EASTERN CAPE |