| Place | Performance | Athlete | Team |
| 1 | 25.69 | Q: JORDAAN - JUANE | FREESTATE |
| 2 | 25.96 | Q: VAN DER MERWE - DENE | NORTHWEST PROVINCE |
| 3 | 26.11 | q: DIEDERICKS - LIENKA | WESTERN CAPE |
| 4 | 26.73 | q: VAN DER MERWE - MIECKE | GAUTENG |
| 5 | 27.50 | -: BOTHA - MARIA CATHARINA | LIMPOPO |
| 6 | 27.85 | -: NEETHLING - DANE | FREESTATE |