| Place | Performance | Athlete | Team |
| 1 | 3:52.45 | Q: PHOLMAN - ANGELL | WESTERN CAPE |
| 2 | 3:55.49 | Q: LEGODI - WINNIE | LIMPOPO |
| 3 | 3:59.20 | Q: HATTIGH - ALRIKE | NORTHWEST PROVINCE |
| 4 | 3:59.65 | Q: VAN DEVENTER - DEMAINE | FREESTATE |
| 5 | 3:59.90 | Q: VERSTER - KARMI | FREESTATE |
| 6 | 4:03.97 | q: PRINSLOO - MIA | EASTERN CAPE |
| 7 | 4:05.58 | q: VILJOEN - EMILY | FREESTATE |
| 8 | 4:08.02 | q: NTSHANGANSE - YAMUKHELA | MPUMALANGA |
| 9 | 4:13.78 | -: NXUMALO - ZAMAZWIDE | KWAZULU-NATAL |
| 10 | 4:14.51 | -: VAN HYSSTEEN - MIA | GAUTENG |
| 11 | 4:25.26 | -: MTHETHWA - NOKWENAMA | KWAZULU-NATAL |
| 12 | 4:26.05 | -: MAMBI - SIYONELA | EASTERN CAPE |