| Place | Performance | Athlete | Team |
| 1 | 4:01.32 | Q: NDABAMBI - IMYOLI | WESTERN CAPE |
| 2 | 4:02.69 | Q: SEBERHAGEN - JANE | GAUTENG |
| 3 | 4:02.73 | Q: JACOBSZ - MISHA | GAUTENG |
| 4 | 4:04.18 | Q: KUBHEKA - ZIKHONA | MPUMALANGA |
| 5 | 4:05.12 | Q: BUTHELEZI - SIPHESIHLE | FREESTATE |
| 6 | 4:07.30 | q: MADIKIZELA - ANAM | EASTERN CAPE |
| 7 | 4:11.44 | q: JINI - SIPHOKUHLE | EASTERN CAPE |
| 8 | 4:12.22 | q: MATTHYS - ELTIANA | WESTERN CAPE |
| 9 | 4:14.48 | -: SESHAUKA - RETHABILE | FREESTATE |
| 10 | 4:32.03 | -: MTHETHWA - APHIWE | KWAZULU-NATAL |
| 11 | 4:39.70 | -: MOHALE - SEANEGO | LIMPOPO |
| 12 | 4:43.07 | -: FERGUSON - IZANIE | MPUMALANGA |