| Place | Performance | Athlete | Team |
| 1 | 24.58 | VERMEULEN - CHANE | RANDBURG |
| 2 | 25.01 | COETZEE(AHMP) - TIMEKE | NORIES UITNODIGINGSPAN |
| 3 | 25.11 | COLENE - SCHEEPERS | MENLOPARK |
| 4 | 26.44 | BOOYSEN(CCA) - JENNA | NORIES UITNODIGINGSPAN |
| 5 | 26.57 | DU PLESSIS - ZANDRE | DRIEHOEK |
| 6 | 26.58 | GROENEWALD - MICHELLE | ZWARTKOP |
| 7 | 26.62 | ESTERHUIZEN - JANINE | TRANSVALIA |
| 8 | 26.66 | LOMBARD - JUNE | WITTEBERG |
| 9 | 26.90 | HOMEN DE GOUEVA - DANIELLA | KEMPTON PARK |
| 10 | 26.92 | GRABE(MNT) - NIAZELL | NORIES UITNODIGINGSPAN |
| 11 | 27.01 | HARVEY - ASHLEY | ELDORAIGNE |
| 12 | 27.13 | KEKANA(SEC) - LERA | NORIES UITNODIGINGSPAN |
| 13 | 27.16 | LAMPRECHT - JOHANE | BASTION |
| 14 | 27.32 | VD BERG(TRV) - SHALAINE | NORIES UITNODIGINGSPAN |
| 15 | 27.50 | BENADE - ANGELIQUE | DRIEHOEK |
| 16 | 27.56 | ROBBERTS(AHMP) - KRISTIN | NORIES UITNODIGINGSPAN |
| 17 | 27.60 | REYNECKE(KPK) - ZANIQUE | NORIES UITNODIGINGSPAN |
| 18 | 27.84 | KOELEMAN - GABBY | CENTURION |
| 19 | 27.95 | DE SWARDT(MNT) - LAN | NORIES UITNODIGINGSPAN |
| 20 | 28.07 | MOKKEN(MPK) - JAYDE | NORIES UITNODIGINGSPAN |
| 21 | 28.44 | LANDSBERG - ABYGAIL | FLORIDA |
| 22 | 28.46 | FRASER - KELLY | VEREENIGING GIMNASIUM |
| 23 | 28.92 | DEAN(MNT) - TEGAN | NORIES UITNODIGINGSPAN |
| 24 | 29.72 | NTOKOZO - MORARE | BIRCHLEIGH |
| 25 | 32.15 | RAMADIBANE - RETHABILE | FOURWAYS |