| Place | Performance | Athlete | Team |
| 1 | 25.93 | FOURIE - ASTRID | VEREENIGING GIMNASIUM |
| 2 | 26.02 | NDUBUEZE - OLUCHI | RANDPARK HIGH |
| 3 | 26.17 | ACHURCH(AHMP) - KAYLIN | NORIES UITNODIGINGSPAN |
| 4 | 26.24 | STRONG(HMK) - DAVIDA | NORIES UITNODIGINGSPAN |
| 5 | 26.41 | DE KLERK - AMAE | HELPMEKAAR |
| 6 | 26.85 | VAN DER MERWE - MIA | RANDBURG |
| 7 | 27.09 | UNGERER - LANA | EG JANSEN |
| 8 | 27.17 | ERASMUS(EHS) - MARLIONE | NORIES UITNODIGINGSPAN |
| 9 | 27.18 | DE BEER(MPK) - IZEL | NORIES UITNODIGINGSPAN |
| 10 | 27.33 | MEYER(MNT) - NICOLE | NORIES UITNODIGINGSPAN |
| 11 | 27.76 | JANSE VAN RENSBURG - CARA | MIDSTREAM |
| 12 | 27.82 | KEYSER - NINA | CENTURION |
| 13 | 28.10 | EKSTEEN - SUMARI | WITTEBERG |
| 14 | 28.11 | KOOPMAN - KAUM | PAREL VALLEI |
| 15 | 28.18 | DORFLING - CRIZELDE | HOOGENHOUT |
| 16 | 28.28 | SWART - DANELL | HOOGENHOUT |
| 17 | 28.33 | GOOSEN - LEZANEY | ALBERTON |
| 18 | 28.43 | GREYLING - MIKAYLA | RANDPARK HIGH |
| 19 | 28.64 | HOLTZHAUSEN - ZOE | MONUMENT |
| 20 | 28.88 | BOSHOFF - LARA | RANDBURG |
| 21 | 28.93 | SULIVAN - NIKITA | PAREL VALLEI |
| 22 | 29.20 | HANEKOM - SYBON | LYDENBURG |
| 23 | 29.68 | VAN HEERDEN(LIN) - MARLENE | NORIES UITNODIGINGSPAN |
| 24 | 30.96 | BEYERS - MIA | HOR VOLKSKOOL POTCHEFSTROOM |