| Place | Performance | Athlete | Team |
| 1 | 0:44.95 | Q: VAN NIEKERK - WAYDE | BOLAND ATHLETICS |
| 2 | 0:45.97 | Q: NZWANZWA - ANELE | ATHLETICS GAUTENG NORTH IND |
| 3 | 0:46.49 | Q: GELDENHUYS - IVAN DANNY | ATHLETICS GAUTENG NORTH |
| 4 | 0:46.99 | -: RAMMUPUDU - RORISANG | LIMPOPO ATHLETICS |
| 5 | 0:47.02 | -: LOTTERING - DARYL | EASTERN PROVINCE ATHLETICS |
| 6 | 0:47.07 | -: JONKER - MARNUS | ATHLETICS CENTRAL NORTH WEST |
| 7 | 0:47.98 | -: MNYAMANA - BULALI | ATHLETICS GAUTENG NORTH IND |
| 8 | 0:48.33 | -: HLATSWAYO - MILDO | KWA-ZULU NATAL ATHLETICS |