| Place | Performance | Athlete | Team |
| 1 | 0:44.94 | Q: PILLAY - LYTHE | CENTRAL GAUTENG ATHLETICS |
| 2 | 0:45.45 | Q: ISAACS - GARDEO | WESTERN PROVINCE ATHLETICS |
| 3 | 0:45.80 | Q: NENE - ZAKITHI | KWA-ZULU NATAL ATHLETICS |
| 4 | 0:45.88 | -: SHEZI - TUMISANG | ATHLETICS GAUTENG NORTH |
| 5 | 0:46.17 | -: SEMENYA - JOEL | ATHLETICS GAUTENG NORTH |
| 6 | 0:47.72 | -: VAN DER WALT - TJ | EASTERN PROVINCE ATHLETICS |
| 7 | 0:47.72 | -: MASHIGO - KARABO | ATHLETICS GAUTENG NORTH IND |
| 8 | 0:49.13 | -: KHUZWAYO - UKHONA | ATHLETICS GAUTENG NORTH IND |