| Place | Performance | Athlete | Team |
| 1 | 23.12 | Q: NEKHUBUI - SHIRLEY | EASTERN PROVINCE ATHLETICS |
| 2 | 23.49 | Q: THOMAS - TAMZIN | ATHLETICS CENTRAL NORTH WEST |
| 3 | 23.76 | Q: AHRENS - KIRSTEN | KWA-ZULU NATAL ATHLETICS |
| 4 | 24.00 | -: LE GRANGE - KAYLA | CENTRAL GAUTENG ATHLETICS |
| 5 | 24.22 | -: LE ROUX - KAYLEE | CENTRAL GAUTENG ATHLETICS |
| 6 | 24.66 | -: COMPAAN - MILA | ATHLETICS GAUTENG NORTH IND |
| 7 | 24.73 | -: MATSHITSE - REABAKA | ATHLETICS GAUTENG NORTH |
| 8 | 24.99 | -: BREEDT - SIMONE | ATHLETICS GAUTENG NORTH IND |