| Place | Performance | Athlete | Team |
| 1 | 24.24 | Q: THOMAS - TAMZIN | ATHLETICS CENTRAL NORTH WEST |
| 2 | 24.59 | Q: MBISHA - JOVIALE | CENTRAL GAUTENG ATHLETICS |
| 3 | 24.85 | Q: COMPAAN - MILA | ATHLETICS GAUTENG NORTH IND |
| 4 | 25.25 | -: MURRAY - TOINETTE | ATHLETICS GAUTENG NORTH IND |
| 5 | 25.47 | -: ROTHMAN - AMIEL | ATHLETICS CENTRAL NORTH WEST |
| 6 | 25.97 | -: SCHOONBEE - BIANCA | EASTERN PROVINCE ATHLETICS |