| Place | Performance | Athlete | Team |
| 1 | 23.56 | Q: NEKHUBUI - SHIRLEY | EASTERN PROVINCE ATHLETICS |
| 2 | 24.49 | Q: EILERD - CHARLIZE | ATHLETICS GAUTENG NORTH IND |
| 3 | 24.57 | Q: LE ROUX - KAYLEE | CENTRAL GAUTENG ATHLETICS |
| 4 | 24.72 | -: MATSHITSE - REABAKA | ATHLETICS GAUTENG NORTH |
| 5 | 24.94 | -: BREEDT - SIMONE | ATHLETICS GAUTENG NORTH IND |
| 6 | 25.61 | -: NGEMA - SINETHEMBA | KWA-ZULU NATAL ATHLETICS |
| 7 | 25.62 | -: BYMAN - LAURE | WESTERN PROVINCE ATHLETICS |
| 8 | 27.24 | -: VAN DER HEEVER - DESMORENE | NORTH WESTERN CAPE ATHLETICS |