| Place | Performance | Athlete | Team |
| 1 | 23.98 | Q: SHABANGU - BANELE | ATHLETICS GAUTENG NORTH |
| 2 | 24.51 | Q: LE GRANGE - KAYLA | CENTRAL GAUTENG ATHLETICS |
| 3 | 24.73 | Q: FOURIE - ANNAME | ATHLETICS FREE STATE |
| 4 | 25.31 | -: VOSLOO - LEONETTE | ATHLETICS GAUTENG NORTH IND |
| 5 | 25.47 | -: VAN SCHALKWYK - IZANEL | ATHLETICS GAUTENG NORTH IND |
| 6 | 25.67 | -: LAUBSCHER - CHARLIZE | ATHLETICS VAAL TRIANGLE |
| 7 | 25.85 | -: OOSTHUIZEN - SYDNEY | ATHLETICS CENTRAL NORTH WEST |