| Place | Performance | Athlete | Team |
| 1 | 23.16 | Q: COETZEE - MIRENDA | ATHLETICS NORTH WEST NORTH |
| 2 | 24.09 | Q: AHRENS - KIRSTEN | KWA-ZULU NATAL ATHLETICS |
| 3 | 24.15 | Q: JOSEPH - ROGAIL | ATHLETICS CENTRAL NORTH WEST |
| 4 | 24.45 | -: BIELDT - TYLON | ATHLETICS GAUTENG NORTH |
| 5 | 24.93 | -: SMIDT - CHARDONE | ATHLETICS VAAL TRIANGLE |
| 6 | 25.43 | -: STOLZ - JENNE | CENTRAL GAUTENG ATHLETICS |
| 7 | 25.52 | -: SEKESE - MPHOTSENG | ATHLETICS GAUTENG NORTH |