| Place | Performance | Athlete | Team |
| 1 | 0:45.95 | Q: PILLAY - LYTHE | CENTRAL GAUTENG ATHLETICS |
| 2 | 0:46.22 | Q: NZWANZWA - ANELE | ATHLETICS GAUTENG NORTH IND |
| 3 | 0:47.25 | Q: RAMMUPUDU - RORISANG | LIMPOPO ATHLETICS |
| 4 | 0:47.64 | -: RAMOTWALA - KGOTHATSO | LIMPOPO ATHLETICS |
| 5 | 0:47.64 | -: DIKGALE - RANTI | ATHLETICS GAUTENG NORTH IND |
| 6 | 0:48.55 | -: MTHIMKULU - MTHI | ATHLETICS FREE STATE |
| 7 | 0:49.27 | -: CONRADIE - PIETER | ATHLETICS GAUTENG NORTH IND |