| Place | Performance | Athlete | Team |
| 1 | 0:45.99 | Q: VAN NIEKERK - WAYDE | BOLAND ATHLETICS |
| 2 | 0:46.37 | Q: NENE - ZAKITHI | KWA-ZULU NATAL ATHLETICS |
| 3 | 0:46.59 | Q: SEMENYA - JOEL | ATHLETICS GAUTENG NORTH |
| 4 | 0:46.91 | q: LOTTERING - DARYL | EASTERN PROVINCE ATHLETICS |
| 5 | 0:46.99 | q: VAN DER WALT - TJ | EASTERN PROVINCE ATHLETICS |
| 6 | 0:47.78 | -: MAHLANGU - SIHLE | ATHLETICS CENTRAL NORTH WEST |
| 7 | 0:49.75 | -: BRIEL - DANIEL | LIMPOPO ATHLETICS |
| 8 | 0:52.54 | -: MILA - MNAMBATHI | ATHLETICS TRANSKEI |