| Place | Performance | Athlete | Team |
| 1 | 0:45.93 | Q: GELDENHUYS - IVAN DANNY | ATHLETICS GAUTENG NORTH |
| 2 | 0:45.94 | Q: ISAACS - GARDEO | WESTERN PROVINCE ATHLETICS |
| 3 | 0:46.16 | Q: JONKER - MARNUS | ATHLETICS CENTRAL NORTH WEST |
| 4 | 0:47.20 | q: MNYAMANA - BULALI | ATHLETICS GAUTENG NORTH IND |
| 5 | 0:49.15 | -: MOHOLOBELA - KABELO | CENTRAL GAUTENG ATHLETICS |
| 6 | 0:49.35 | -: MOTLOBA - KAMOGELO | ATHLETICS NORTH WEST NORTH |
| 7 | 0:50.77 | -: DE WEE - MYRON | NORTH WESTERN CAPE ATHLETICS |
| 8 | 0:51.66 | -: MAKOBE - TSHEPISO | ATHLETICS VAAL TRIANGLE |