| Place | Performance | Athlete | Team |
| 1 | 0:53.70 | Q: VERMEULEN - CHANE | CENTRAL GAUTENG ATHLETICS |
| 2 | 0:54.08 | Q: STRIJDOM - ANGELIQUE | ATHLETICS GAUTENG NORTH IND |
| 3 | 0:54.48 | q: MOLEPO - PRECIOUS | ATHLETICS GAUTENG NORTH |
| 4 | 0:55.28 | -: DE WET - SIMONE | ATHLETICS GAUTENG NORTH IND |
| 5 | 0:55.93 | -: NAUDE - AMY | KWA-ZULU NATAL ATHLETICS |
| 6 | 0:56.09 | -: DUBE - FINA | CENTRAL GAUTENG ATHLETICS |
| 7 | 0:56.89 | -: THOMSEN - MICHAELA | ATHLETICS CENTRAL NORTH WEST |
| 8 | 0:58.44 | -: ACKERMAN - LIZE | ATHLETICS FREE STATE |