| Place | Performance | Athlete | Team |
| 1 | 0:43.55 | RELAY TEAM - | WESTERN CAPE MAINSTREAM |
| 2 | 0:44.35 | RELAY TEAM - | GAUTENG MAINSTREAM |
| 3 | 0:45.19 | RELAY TEAM - | MPUMALANGA MAINSTREAM |
| 4 | 0:45.45 | RELAY TEAM - | FREE STATE MAINSTREAM |
| 5 | 0:46.57 | RELAY TEAM - | KWAZULU NATAL MAINSTREAM |
| 6 | 0:47.78 | RELAY TEAM - | LIMPOPO MAINSTREAM |