| Place | Performance | Athlete | Team |
| 1 | 23.97 | LE ROUX - KAYLEE | GAUTENG MAINSTREAM |
| 2 | 24.32 | LA GRANGE - KAYLA | GAUTENG MAINSTREAM |
| 3 | 24.72 | BOOYSEN - MIENKE | MPUMALANGA MAINSTREAM |
| 4 | 24.78 | OOSTHUIZEN - SYDNEY | FREE STATE MAINSTREAM |
| 5 | 25.01 | SCHOONBEE - BIANCA | EASTERN CAPE MAINSTREAM |
| 6 | 25.35 | DE KOCK - ASHTON | GAUTENG MAINSTREAM |
| 7 | 25.40 | BEUKES - ANIKA | WESTERN CAPE MAINSTREAM |
| 8 | 25.91 | KOTZE - DANE | GAUTENG MAINSTREAM |