| Place | Performance | Athlete | Team |
| 1 | 24.09 | BOTJE - KAILI | GAUTENG MAINSTREAM |
| 2 | 24.19 | VERMEULEN - CHANE | GAUTENG MAINSTREAM |
| 3 | 24.76 | WANSBURY - CHARNE | FREE STATE MAINSTREAM |
| 4 | 25.14 | VAN NIEKERK - TENINKE | MPUMALANGA MAINSTREAM |
| 5 | 25.18 | ELLIOT - AMBER | EASTERN CAPE MAINSTREAM |
| 6 | 25.45 | EZE - DIANA | GAUTENG MAINSTREAM |
| 7 | 25.62 | VAN HEERDEN - JESICCA | NORTH WEST MAINSTREAM |
| 8 | 25.76 | DE BEER - MIA | NORTH WEST MAINSTREAM |