| Place | Performance | Athlete | Team |
| 1 | 43.50 | WILSON - MIA | WESTERN CAPE MAINSTREAM |
| 2 | 43.99 | VISSER - CARLI | NORTH WEST MAINSTREAM |
| 3 | 44.70 | RAATH - CASSANDRA | GAUTENG MAINSTREAM |
| 4 | 44.73 | PIENAAR - DANYELLE | FREE STATE MAINSTREAM |
| 5 | 45.82 | VERSTER - LEA | NORTH WEST MAINSTREAM |
| 6 | 46.02 | ENGELBRECHT - CHRISTINA ALIDA | WESTERN CAPE MAINSTREAM |
| 7 | 46.36 | JENKINS - CAYLEN | GAUTENG MAINSTREAM |
| 8 | 50.87 | TEN CATE - AMY | WESTERN CAPE MAINSTREAM |