| Place | Performance | Athlete | Team |
| 1 | 28.86 | Q: SWARTS - CAITLIN | WESTERN CAPE LSEN |
| 2 | 29.18 | Q: LINKS - ANNASTASIA A N | FREE STATE LSEN |
| 3 | 29.48 | Q: JEFFRIES - JEZLIN | GAUTENG LSEN |
| 4 | 29.72 | q: VILANKULA - AMKELANI | MPUMALANGA LSEN |
| 5 | 30.23 | q: SPOGTER - LESLEY ANN | WESTERN CAPE LSEN |
| 6 | 33.13 | -: MURARE - TANAKA | GAUTENG LSEN |