| Place | Performance | Athlete | Team |
| 1 | 23.11 | q: VAN ECK - REAVANO N | FREE STATE LSEN |
| 2 | 23.19 | q: MANELI - LOXOLO | GAUTENG LSEN |
| 3 | 23.48 | q: ZULU - NKOSINATHI | MPUMALANGA LSEN |
| 4 | 23.57 | q: VAALTYN - ETHAN | EASTERN CAPE LSEN |
| 5 | 24.11 | -: NAGEL - LERENZO M | LIMPOPO LSEN |
| 6 | 24.33 | -: DE BEER - CHRISTO | MPUMALANGA LSEN |