| Place | Performance | Athlete | Team |
| 1 | 22.36 | q: SEPTEMBER - ELDRIO | WESTERN CAPE LSEN |
| 2 | 22.83 | q: OLIPHANT - HAYGAN | GAUTENG LSEN |
| 3 | 23.67 | -: SINDANE - SIPHO | MPUMALANGA LSEN |
| 4 | 23.69 | -: NKUNA - NEO | NORTH WEST LSEN |
| 5 | 23.96 | -: THATHLEDI - ORABILE | GAUTENG LSEN |
| 6 | 24.92 | -: MANGISA - XHANTI | EASTERN CAPE LSEN |
| 7 | 26.31 | -: RADEBE - KARABO | FREE STATE LSEN |