| Place | Performance | Athlete | Team |
| 1 | 29.34 | q: BIANCHINA - CHANTEL | FREE STATE LSEN |
| 2 | 29.52 | q: MOLOI - LERATO | FREE STATE LSEN |
| 3 | 29.60 | q: VAN DEN HEEVER - JAMIE-LEE | GAUTENG LSEN |
| 4 | 30.76 | -: ZWARTS - BIANCA | GAUTENG LSEN |
| 5 | 33.07 | -: SANDERS - NATALIE | KWAZULU NATAL LSEN |