| Place | Performance | Athlete | Team |
| 1 | 29.11 | q: ISAACS - JEAN | WESTERN CAPE LSEN |
| 2 | 29.21 | q: BUYS - JUANITA | FREE STATE LSEN |
| 3 | 29.66 | q: MOILA - KATLEGE | GAUTENG LSEN |
| 4 | 31.30 | -: MAKOBE - KATLEGO | MPUMALANGA LSEN |
| 5 | 31.49 | -: ROETS - MIA | GAUTENG LSEN |
| 6 | 31.81 | -: NEL - JAZMIN | EASTERN CAPE LSEN |