| Place | Performance | Athlete | Team |
| 1 | 23.14 | Q: JANSEN VAN VUUREN - DONOVAN | GAUTENG LSEN |
| 2 | 23.77 | Q: JANSEN - RAYLEN-LEE | WESTERN CAPE LSEN |
| 3 | 24.31 | q: ARENDS - RENALDO | WESTERN CAPE LSEN |
| 4 | 25.04 | q: SEFIKA - RETHABILE | GAUTENG LSEN |
| 5 | 26.19 | -: LANGLEY - KAY-DINN JF | FREE STATE LSEN |
| 6 | 26.44 | -: SKOSANA - SIPHESIHLE | MPUMALANGA LSEN |
| 7 | 27.05 | -: LUKHELE - LUNGA | MPUMALANGA LSEN |