| Place | Performance | Athlete | Team |
| 1 | 21.59 | Q: NTSEPELA - PHILASANDE | MPUMALANGA MAINSTREAM |
| 2 | 21.73 | Q: MARTIN - WIAAN | WESTERN CAPE MAINSTREAM |
| 3 | 21.78 | Q: OLIPHANT - DAYLAN | NORTH WEST MAINSTREAM |
| 4 | 22.02 | q: POTGIETER - ANDR | MPUMALANGA MAINSTREAM |
| 5 | 22.21 | q: DUBE - KHAYA | MPUMALANGA MAINSTREAM |
| 6 | 22.48 | -: RIKHOTSO - MBUYELO | MPUMALANGA MAINSTREAM |
| 7 | 22.91 | -: ZWANE - MOFIKI | FREE STATE MAINSTREAM |