| Place | Performance | Athlete | Team |
| 1 | 24.95 | Q: LE ROUX - KAYLEE | GAUTENG MAINSTREAM |
| 2 | 25.03 | Q: LA GRANGE - KAYLA | GAUTENG MAINSTREAM |
| 3 | 25.29 | Q: OOSTHUIZEN - SYDNEY | FREE STATE MAINSTREAM |
| 4 | 25.53 | q: SCHOONBEE - BIANCA | EASTERN CAPE MAINSTREAM |
| 5 | 25.57 | q: BEUKES - ANIKA | WESTERN CAPE MAINSTREAM |
| 6 | 25.73 | -: EDWARDS - CHANE | NORTH WEST MAINSTREAM |
| 7 | 27.68 | -: PLAATJIES - MAXINE | NORTHERN CAPE MAINSTREAM |
| 8 | 28.73 | -: GUMEDE - SNENHLANHLA | KWAZULU NATAL MAINSTREAM |