| Place | Performance | Athlete | Team |
| 1 | 22.04 | Q: LABUSCHAGNE - JANES | NORTHERN CAPE MAINSTREAM |
| 2 | 22.28 | Q: FLUKS - COLE | WESTERN CAPE MAINSTREAM |
| 3 | 22.29 | Q: MASINA - SIYABONGA | MPUMALANGA MAINSTREAM |
| 4 | 22.30 | q: RAMMUDU - RORISANG | LIMPOPO MAINSTREAM |
| 5 | 22.50 | -: VAJSEL - ANTONIO | NORTH WEST MAINSTREAM |
| 6 | 22.60 | -: STAFA - LUKHANYO DANZELL | FREE STATE MAINSTREAM |
| 7 | 22.92 | -: LE ROUX - JACOBUS FRANCOIS | FREE STATE MAINSTREAM |