| Place | Performance | Athlete | Team |
| 1 | 25.56 | Q: VAN HEERDEN - JESICCA | NORTH WEST MAINSTREAM |
| 2 | 25.58 | Q: EZE - DIANA | GAUTENG MAINSTREAM |
| 3 | 26.66 | -: GOVENDER - TIARA | KWAZULU NATAL MAINSTREAM |
| 4 | 26.89 | -: VAN ASWEGEN - DOMINIQUE | MPUMALANGA MAINSTREAM |
| 5 | 27.79 | -: SLABBERT - LENE | NORTHERN CAPE MAINSTREAM |
| 6 | 28.14 | -: MATLOU - KAMAGELO | LIMPOPO MAINSTREAM |