| Place | Performance | Athlete | Team |
| 1 | 25.03 | Q: VERMEULEN - CHANE | GAUTENG MAINSTREAM |
| 2 | 25.34 | Q: VAN NIEKERK - TENINKE | MPUMALANGA MAINSTREAM |
| 3 | 25.80 | -: DU PLESSIS - ZANDRE | GAUTENG MAINSTREAM |
| 4 | 26.02 | -: SMIT - ZAHNI | WESTERN CAPE MAINSTREAM |
| 5 | 26.62 | -: RAMPALENG - JUSTINE PABALELO | FREE STATE MAINSTREAM |
| 6 | 27.02 | -: UYS - ULANI | NORTHERN CAPE MAINSTREAM |
| 7 | 27.68 | -: BABALWA - DICKSON | LIMPOPO MAINSTREAM |