| Place | Performance | Athlete | Team |
| 1 | 24.64 | Q: WANSBURY - CHARNE | FREE STATE MAINSTREAM |
| 2 | 24.74 | Q: BOTJE - KAILI | GAUTENG MAINSTREAM |
| 3 | 25.48 | q: ELLIOT - AMBER | EASTERN CAPE MAINSTREAM |
| 4 | 25.55 | q: DE BEER - MIA | NORTH WEST MAINSTREAM |
| 5 | 26.79 | -: TERBLANCHE - TIA | KWAZULU NATAL MAINSTREAM |
| 6 | 28.73 | -: MORRIS - CYAL-LEE | NORTHERN CAPE MAINSTREAM |
| 7 | 29.74 | -: MAAKE - RATSIBE | LIMPOPO MAINSTREAM |