| Place | Performance | Athlete | Team |
| 1 | 25.98 | Q: DE KLERK - AMAE | GAUTENG MAINSTREAM |
| 2 | 26.73 | Q: BOOYSEN - TAMRYN | WESTERN CAPE MAINSTREAM |
| 3 | 27.40 | -: REINSTEIN - NOA | KWAZULU NATAL MAINSTREAM |
| 4 | 28.05 | -: VAN NIEKERK - AMELIE | NORTHERN CAPE MAINSTREAM |
| 5 | 28.68 | -: BOOYSEN - EUCARIA | NORTHERN CAPE MAINSTREAM |