| Place | Performance | Athlete | Team |
| 1 | 25.04 | Q: LATEGAN - MEGAN | GAUTENG MAINSTREAM |
| 2 | 25.39 | Q: ESTERHUYSE - VEN | MPUMALANGA MAINSTREAM |
| 3 | 25.61 | q: BANUTELO - BAGINYO KETSIA | FREE STATE MAINSTREAM |
| 4 | 26.10 | -: CROUS - ANE | FREE STATE MAINSTREAM |
| 5 | 26.23 | -: LAMBRECHT - IZAAN | EASTERN CAPE MAINSTREAM |
| 6 | 27.44 | -: MARAIS - ERYNNE | KWAZULU NATAL MAINSTREAM |
| 7 | 27.50 | -: NOVELA - MONICA | LIMPOPO MAINSTREAM |