| Place | Performance | Athlete | Team |
| 1 | 25.17 | Q: VAN OUDTSHOORN - KAYLIE | GAUTENG MAINSTREAM |
| 2 | 25.97 | Q: UDENZE - CHISOM SHANTE | WESTERN CAPE MAINSTREAM |
| 3 | 25.99 | q: BURGER - RUME | FREE STATE MAINSTREAM |
| 4 | 26.10 | -: CILLIERS - ZOë | MPUMALANGA MAINSTREAM |
| 5 | 26.38 | -: FICK - ELIZABETH | FREE STATE MAINSTREAM |
| 6 | 26.75 | -: LEBEPE - MAITE | LIMPOPO MAINSTREAM |