| Place | Performance | Athlete | Team |
| 1 | 0:41.45 | RELAY TEAM - | NORTH WEST MAINSTREAM |
| 2 | 0:41.82 | RELAY TEAM - | MPUMALANGA MAINSTREAM |
| 3 | 0:42.22 | RELAY TEAM - | WESTERN CAPE MAINSTREAM |
| 4 | 0:42.55 | RELAY TEAM - | GAUTENG MAINSTREAM |
| 5 | 0:42.57 | RELAY TEAM - | FREE STATE MAINSTREAM |
| 6 | 0:44.03 | RELAY TEAM - | NORTHERN CAPE MAINSTREAM |
| 7 | 0:44.95 | RELAY TEAM - | EASTERN CAPE MAINSTREAM |