| Place | Performance | Athlete | Team |
| 1 | 0:53.81 | Q: VAN DER MERWE - HANDR | MPUMALANGA MAINSTREAM |
| 2 | 0:55.44 | Q: DLAMINI - EVINCE | GAUTENG MAINSTREAM |
| 3 | 0:55.66 | Q: MUCHANGA - ISAAC | NORTH WEST MAINSTREAM |
| 4 | 0:56.43 | q: PARKIN - REECE | GAUTENG MAINSTREAM |
| 5 | 0:58.91 | -: FRIEDBURG - BENO-WAYNE | NORTHERN CAPE MAINSTREAM |
| 6 | 0:59.87 | -: SCHEEPERS - MORNE | WESTERN CAPE MAINSTREAM |