| Place | Performance | Athlete | Team |
| 1 | 0:54.23 | Q: ALBLAS - EDRICH | GAUTENG MAINSTREAM |
| 2 | 0:55.47 | Q: VAN RENSBURG - WERNICH | EASTERN CAPE MAINSTREAM |
| 3 | 0:55.62 | Q: CAPAZORIO - MARCO | MPUMALANGA MAINSTREAM |
| 4 | 0:56.10 | q: HARACIDO - DANNY | GAUTENG MAINSTREAM |
| 5 | 0:57.66 | -: BESTER - TIAAN | LIMPOPO MAINSTREAM |
| 6 | 0:58.13 | -: GXIYA - NCEBA PRETTYBOY | FREE STATE MAINSTREAM |
| 7 | 1:02.10 | -: SIBIYA - PHILABANDE | MPUMALANGA MAINSTREAM |