| Place | Performance | Athlete | Team |
| 1 | 0:53.21 | Q: BERNING - TYRONE | MPUMALANGA MAINSTREAM |
| 2 | 0:53.91 | Q: V.D.LITH - ZANDER | GAUTENG MAINSTREAM |
| 3 | 0:53.95 | Q: HATTINGH - JUSTINE | GAUTENG MAINSTREAM |
| 4 | 0:53.99 | q: NKANYONI - NTIYSIO | MPUMALANGA MAINSTREAM |
| 5 | 0:54.08 | q: BOTHMA - BRANDON LEIGH | WESTERN CAPE MAINSTREAM |
| 6 | 0:55.10 | -: BROODRYK - GERT HENDRIK | FREE STATE MAINSTREAM |
| 7 | 0:56.12 | -: SEBOLAO - PULE | LIMPOPO MAINSTREAM |
| 8 | 0:56.26 | -: VAN DER HEEVER - JORDAN KURT | WESTERN CAPE MAINSTREAM |