| Place | Performance | Athlete | Team |
| 1 | 0:53.71 | Q: NEL - CLEMOND THEMBANI | WESTERN CAPE MAINSTREAM |
| 2 | 0:53.74 | Q: FRAZER - WJ | MPUMALANGA MAINSTREAM |
| 3 | 0:53.88 | Q: MALULEKE - UTHANDILE | NORTH WEST MAINSTREAM |
| 4 | 0:54.40 | -: SHALE - ORATILE | GAUTENG MAINSTREAM |
| 5 | 0:54.73 | -: ENGELBRECHT - ROELOF | KWAZULU NATAL MAINSTREAM |
| 6 | 0:54.83 | -: PIETERSE - JUSTIN | GAUTENG MAINSTREAM |
| 7 | 0:56.48 | -: DU PLESSIS - JUAN | EASTERN CAPE MAINSTREAM |
| 8 | 0:58.46 | -: FISCHER - CALEB | NORTHERN CAPE MAINSTREAM |