| Place | Performance | Athlete | Team |
| 1 | 1:05.10 | Q: MATTHYSEN - NIKE | GAUTENG MAINSTREAM |
| 2 | 1:05.29 | Q: KOTZE - JUANNE | FREE STATE MAINSTREAM |
| 3 | 1:06.44 | Q: VAN ZYL - LUANDI | LIMPOPO MAINSTREAM |
| 4 | 1:07.31 | q: VAN WYK - ZANDR | MPUMALANGA MAINSTREAM |
| 5 | 1:07.55 | -: YOUNG - GISELE | WESTERN CAPE MAINSTREAM |