| Place | Performance | Athlete | Team |
| 1 | 1:04.33 | Q: SAAIMAN - HAILEY | MPUMALANGA MAINSTREAM |
| 2 | 1:05.52 | Q: CARROLL - LIENKE | NORTH WEST MAINSTREAM |
| 3 | 1:05.80 | Q: SWART - RIANA | GAUTENG MAINSTREAM |
| 4 | 1:06.13 | q: MOKOLWANE - REABETSE | GAUTENG MAINSTREAM |
| 5 | 1:10.27 | -: VENTER - JANE | EASTERN CAPE MAINSTREAM |