| Place | Performance | Athlete | Team |
| 1 | 1:02.71 | Q: DE KOCK - BARBARA JEAN | WESTERN CAPE MAINSTREAM |
| 2 | 1:03.09 | Q: SPERSHOT - GERMARIE | FREE STATE MAINSTREAM |
| 3 | 1:03.51 | Q: BREEDT - LU CHELLE | GAUTENG MAINSTREAM |
| 4 | 1:04.25 | q: MARX - LEE-ANN | NORTH WEST MAINSTREAM |
| 5 | 1:05.08 | q: BLACK - ZANRI | EASTERN CAPE MAINSTREAM |
| 6 | 1:06.94 | -: STEENKAMP - MIA | MPUMALANGA MAINSTREAM |