| Place | Performance | Athlete | Team |
| 1 | 39.34 | Q: PIENAAR - ALEX | GAUTENG MAINSTREAM |
| 2 | 39.84 | Q: ENGELBRECHT - MORNE | KWAZULU NATAL MAINSTREAM |
| 3 | 41.17 | q: LETSOALO - TUMISO | MPUMALANGA MAINSTREAM |
| 4 | 41.52 | -: BONTHUYS - ADRIAAN | FREE STATE MAINSTREAM |
| 5 | 42.06 | -: MAFATA - KGOTSO | MPUMALANGA MAINSTREAM |
| 6 | 44.85 | -: MAKHANDA - PIET | LIMPOPO MAINSTREAM |